Laboratory of Single-Molecule Cell Biology, Kyoto Univ. Grad. Sch. Of Biostudies
Department of Pharmacology, Kyoto University Faculty of Medicine

Title Pictures

Live-cell fluorescence single-molecule imaging just emerged in the beginning of this century. Our laboratory aims at extending this potent approach of viewing specific molecular events in living cells. "Seeing (or thinking) single-molecule is believing."

What's New

  • Mar. 24, 2021    Our paper describing a mechanism of paradoxical cell proliferation promoted by anti-cancer kinase inhibitors is published in Cell Reports. (Link)
  • Feb. 2, 2021    Our paper describing a method for development of fluorescent probes for super-resolution microscopy, IRIS is published in Cell Reports. (Link) (Journal Cover)
  • Aug. 1, 2020    Dr. Eiji Usukura joined our laboratory (Program-Specific Assistant Professor for NIH joint research project).
  • Jan. 8, 2019    Our paper describing convection-induced biased misdistribution of actin probes is published in Biophysical Journal.(Link) (Link2).Our related paper is also published in Molecular Biology of the Cell (Aug. 2018, Link)
  • May. 14, 2018    Our paper describing the actin filament untwisting by the rotational polymerization by mDia1 is published in PNAS. (Open Access) (Link2) (Link3)
  • Apr. 1, 2018    Dr. Akitoshi Miyamoto joined our laboratory (Assistant Professor as of May 1, 2018).
  • Mar. 6, 2018    We hold a celebration for Dr. Shuh Narumiya who was designated as Bunkakoro-sha (Person of Cultural Merit). (Photos (password required))
  • Mar. 1, 2018    Dr. Sawako Yamashiro is promoted as Lecture in Kyoto University Graduate School of Biostudies.
  • Mar. 7, 2016    Associate Professor Tai Kiuchi received the Nakatani Award (Incentive Prize).
  • Jul. 6, 2015    Our paper introducing new concept multitarget super-resolution microscopy, IRIS, has been published in Nature Methods. By employing exchangeable fluorescent probes that bind the target transiently, IRIS yields densely-labeled high-fidelity images of the target structures, which is a major breakthrough for molecular localization studies at 10 nanometer scale. (Link)
  • Apr. 1, 2015    “Laboratory of Single-Molecule Cell Biology” is launched as the new name for our laboratory in Graduate School of Biostudies.
  • Jan. 8, 2015    A detailed protocol for our eSiMS microscopy has been published. eSiMS stands for “easy, efficient and electroporation-based Single-Molecule Speckle” microscopy. (Link)
  • Jan. 1, 2015    Our laboratory joined Kyoto University Graduate School of Biostudies in addition to Faculty of Medicine.
  • Jul. 1, 2014    We have moved from Tohoku University to Kyoto University. Our laboratory organizes Department of Pharmacology, Kyoto University Faculty Medicine where Prof. Watanabe used to work.
  • Feb. 21, 2014    A new paper by Yamashiro et al. is accepted for publication in MBoC. In this article, we introduce a new easy-to-use single-molecule speckle (SiMS) microscopy which enables nanometer-scale flow velocity and wide-range lifetime measurement of cellular actin filaments. We discovered gathering and collecting actions of focal adhesions on the lamellipodial actin flow. (Link)
  • Jul. 18, 2013    The publication list has been updated with Higashida et al. (Nature Cell Biology, 15, 395-405, 2013) .
  • Jul. 10, 2012    The article on our collaboration with Dr. Vavylonis Group appeared on the Lehigh University Web site. (Link)
  • Feb. 2012    A basic procedure of live-cell single-molecule speckle microscopy is published in Meth. Enzymol. (Link)
  • Oct. 20, 2011    A paper by Masahiro Maruoka et al. has been accepted in Biochemical Journal. (Link)
  • Oct. 5, 2011    A paper by Smith et al., which was supported by the HFSP grant, is published in Biophysical Journal. An ImageJ plugin for particle tracking, ‘Speckle TrackerJ’ is available at
  • Jul. 4, 2011    Sawako Yamashiro joined our laboratory as a postdoc.
  • Apr. 18, 2011    An article by Tai Kiuchi et al. is published in Journal of Cell Biology.
  • Apr. 1, 2011    Tai Kiuchi joined our laboratory as a Research Associate.
  • Feb. 7, 2011    A paper in which Masahiro Maruoka is the corresponding author is published in FEBS Letters. (Pubmed)
  • Jan. 7, 2011    A paper by Hiroaki Mizuno et al. is published in Science.
Kyoto University Faculty of Medicine Graduate School of Biostudies