Yamashiro, S., Rutkowski, D.M., Lynch, K.A., Liu, Y., Vavylonis, D. and Watanabe, N. Force transmission by retrograde actin flow-induced dynamic molecular stretching of Talin. Nat. Commun. 14: 8468 (2023). doi.org/10.1038/s41467-023-44018-z
Zhang, Q., Miyamoto, A. and Watanabe, N. Protocol to generate fast-dissociating recombinant antibody fragments for multiplexed super-resolution microscopy. STAR Protocols 4: 102523 (2023). doi: 10.1016/j.xpro.2023.102523
Watanabe, N. Brownian ratchet force sensor at the contacting point between F-actin barbed end and lamellipodium tip plasma membrane. “Plasma Membrane Shaping” Elsevier 295-306 (2023). doi: 10.1016/B978-0-323-89911-6.00015-7
2022
Zhang, Q., Miyamoto, A., Watanabe, S., Arimori, T., Sakai, M., Tomisaki, M., Kiuchi, T., Takagi, J. and Watanabe, N. Engineered fast-dissociating antibody fragments for multiplexed super-resolution microscopy. Cell Reports Methods 2: 100301 (2022). doi: 10.1016/j.crmeth.2022.100301
Holz, D., Hall, A.R., Usukura, E., Yamashiro, S., Watanabe, N. and Vavylonis, D. A mechanism with severing near barbed ends and annealing explains structure and dynamics of dendritic actin networks. eLife 11: e69031 (2022). doi: 10.7554/eLife.69031
2021
Miyoshi, T., Friedman, T.B. and Watanabe, N. Fast-dissociating but highly specific antibodies are novel tools in biology, especially useful for multiplex super-resolution microscopy. STAR Protocols 2: 100967 (2021).
Higuchi, M., Ishiyama, K., Maruoka, M., Kanamori, R.,Takanori-Kondo, A. and Watanabe, N. Paradoxical activation of c-Src as a drug-resistant mechanism. Cell Rep. 34: 108876 (2021). doi: 10.1016/j.celrep.2021.108876
Miyoshi, T., Zhang, Q., Miyake, T., Watanabe, S.,Ohnishi, H.,Chen, J., Vishwasrao, H.D., Chakraborty, O., Belyantseva, I.A., Perrin, B.J., Shroff, H., Friedman, T.B., Omori, K. and Watanabe, N. Semi-automated single-molecule microscopy screening of fast-dissociating specific antibodies directly from hybridoma cultures. Cell Rep. 34: 108708 (2021). doi: 10.1016/j.celrep.2021.108708
2020
Thumkeo, D., Katsura, Y., Nishimura, Y., Kanchanawong, P., Tohyama, K., Ishizaki, T., Kitajima, S., Takahashi, C., Hirata, T., Watanabe, N., Krummel, M. F. and Narumiya, S. mDia1/3-dependent actin polymerization spatiotemporally controls LAT phosphorylation by Zap70 at the immune synapse.Science Advances 6: eaay2432 (2020). doi: 10.1126/sciadv.aay2432
Yamashiro, S. and Watanabe, N. Quantitative high-precision imaging of myosin-dependent filamentous actin dynamics.J. Muscle Res. Cell Motil. 41: 163-173 (2020). doi: 10.1007/s10974-019-09541-x.
2019
Koseki, K., Taniguchi, D., Yamashiro, S., Mizuno, H., Vavylonis, D. and Watanabe, N. Lamellipodium tip actin barbed ends serve as a force sensor. Genes Cells 24: 705-718 (2019). doi:10.1111/gtc.12720.
Li, H., Miki, T., Almeida, G.M., Hanashima, C., Matsuzaki, T., Kuo, C.J., Watanabe, N. and Noda, M. RECK in neural precursor cells plays a critical role in mouse forebrain angiogenesis. iScience 19: 559-571 (2019). doi: 10.1016/j.isci.2019.08.009.
Yamashiro, S., Taniguchi, D., Tanaka, S., Kiuchi, T., Vavylonis, D. and Watanabe, N. Convection-Induced Biased Distribution of Actin Probes in Live Cells. Biophys. J. 116: 142-150 (2019). doi:10.1016/j.bpj.2018.11.022
2018
Watanabe, N., Tohyama, K. and Yamashiro, S. Mechanostress resistance involving formin homology proteins: G- and F-actin homeostasis-driven filament nucleation and helical polymerization-mediated actin polymer stabilization (review) Biochem. Biophys. Res. Commun. 506: 323-329 (2018). doi: 10.1016/j.bbrc.2018.09.189.
Sakamoto, S., Thumkeo, D., Ohta, H., Zhang, Z., Huang, S., Kanchanawong, P., Fuu, T., Watanabe, S., Shimada, K., Fujihara, Y., Yoshida, S., Ikawa, M., Watanabe, N., Saitou, M. and Narumiya, S. mDia1/3 generate cortical F-actin meshwork in Sertoli cells that is continuous with contractile F-actin bundles and indispensable for spermatogenesis and male fertility. PLoS Biol. 16: e2004874 (2018). doi: 10.1371/journal.pbio.2004874.
Kawabata-Galbraith, K., Fujishima, K., Mizuno, H., Lee, S. J., Uemura, T., Sakimura, K., Mishina, M., Watanabe, N. and Kengaku, M. MTSS1 regulation of actin-nucleating formin DAAM1 in dendritic filopodia determines final dendritic configuration of Purkinje cells. Cell Rep. 24: 95-106 (2018). doi: 10.1016/j.celrep.2018.06.013
Yamashiro, S., Tanaka, S., McMillen, L.M., Taniguchi, D., Vavylonis, D. and Watanabe, N. Myosin-dependent actin stabilization as revealed by single-molecule imaging of actin turnover. Mol. Biol. Cell 29: 1941-1947 (2018). doi: 10.1091/mbc.E18-01-0061.
Mizuno, H., Tanaka, K., Yamashiro, S., Narita, A. and Watanabe, N. Helical rotation of mDia1 generates actin filaments resistant to cofilin. Proc. Natl. Acad. Sci. USA 115: E5000-E5007 (2018). doi: 10.1073/pnas.1803415115.
2017
Ryan, G.L., Holz, D., Yamashiro, S., Taniguchi, D., Watanabe, N. and Vavylonis, D. Cell protrusion and retraction driven by fluctuations in actin polymerization: A two-dimensional model. Cytoskeleton (Hoboken) 74: 490-503 (2017). doi: 10.1002/cm.21389.
Yamashiro, S.. and Watanabe, N.. Overview of single-molecule speckle (SiMS) microscopy and its electroporation-based version with efficient labeling and improved spatiotemporal resolution. Sensors (Basel) 17: E1585 (2017). doi: 10.3390/s17071585
Yamashiro, S.. and Watanabe, N.. An infrared actin probe for deep-cell electroporation-based single-molecule speckle (eSiMS) microscopy. Sensors (Basel) 17: E1545 (2017). doi: 10.3390/s17071545.
2016
Ueyama, T., Nishio, S., Miyoshi, T., Torii, H., Nishimura, K., Sugahara, K., Sakata, H., Thumkeo, D., Sakaguchi, H., Watanabe, N., Usami, S., Saito, N. and Kitajiri, S. Constitutive activation of DIA1 (DIAPH1) via C-terminal truncation causes human sensorineural hearing loss. EMBO Mol. Med. 8: 1310-1324 (2016).
2015
Kiuchi, T., Higuchi, M., Takamura, A., Maruoka, M. and Watanabe, N. Multitarget super-resolution microscopy with high-density labeling by exchangeable probes. Nat. Methods 12: 743-746 (2015). doi: 10.1038/nmeth.3466.
Yamashiro, S., Mizuno, H.. and Watanabe, N. An easy-to-use single-molecule speckle microscopy enabling nanometer-scale flow and wide-range lifetime measurement of cellular actin filaments. Methods Cell Biol. 125: 43-59 (2015).
2014
Yamashiro, S. and Watanabe, N. A new link between the retrograde actin flow and focal adhesions. (JB Review) J. Biochemistry 156: 239-248 (2014)
Kiuchi, T., Ortiz-Zapater, E., Monypenny, J., et al. The ErbB4 CYT2 variant protects EGFR from ligand-induced degradation to enhance cancer cell motility. Science Signaling 7: ra78 (2014).
Yamashiro, S., Mizuno, H., Smith, M. B., Ryan, G. L., Kiuchi, T. Vavylonis, D. and Watanabe, N. New single-molecule speckle microscopy reveals modification of the retrograde actin flow by focal adhesions at nanometer scales. Mol. Biol. Cell 125: 43-59 (2014).
Mizuno, H. and Watanabe, N. Rotational movement of formins evaluated by using single-molecule fluorescence polarization. Meth. Enzymol. 540: 73-94 (2014)
2013
Watanabe, N., Yamashiro, S., Vavylonis, D. and Kiuchi, T. Molecular viewing of actin polymerizing actions and beyond: Combination analysis of single-molecule speckle microscopy with modeling, FRAP and s-FDAP (sequential fluorescence decay after photoactivation). (Review) Dev. Growth Differ. 55: 508–514 (2013).
Higashida, C., Kiuchi, T., Akiba, Y., Mizuno, H., Maruoka, M., Narumiya, S., Mizuno, K. and Watanabe, N. F- and G-actin homeostasis regulates mechanosensitive actin nucleation by formins. Nat. Cell Biol. 15: 395-405 (2013).
Miyoshi, T. and Watanabe, N. Can filament treadmilling alone account for the F-actin turnover in lamellipodia? Cytoskeleton (Hoboken) 70: 179–190 (2013)
Smith, M.B., Kiuchi, T., Watanabe, N. and Vavylonis, D. Distributed actin turnover in the lamellipodium and FRAP kinetics. Biophys. J. 104: 247-257 (2013)
Ryan, G. L., Watanabe, N. and Vavylonis, D. Image analysis tools to quantify cell shape and protein dynamics near the leading edge. Cell Struct. Funct. 38: 1-7 (2013)
2012
Watanabe, N. Fluorescence single-molecule imaging of actin turnover and regulatory mechanisms. Meth. Enzymol. 505, 219-232 (2012)
Koizumi, K., Takano, K., Kaneyasu, A., Watanabe-Takano, H., Tokuda, E., Abe, T., Watanabe, N., Takenawa, T. and Endo, T. RhoD activated by fibroblast growth factor induces cytoneme-like cellular protrusions through mDia3C. Mol. Biol. Cell 23: 4647-4661 (2012).
Mizuno, H. and Watanabe, N. mDia1 and formins: screw cap of the actin filament. (Review) Biophysics, 8: 95-102 (2012)
Murata, K., Kitaori, T., Oishi, S., Watanabe, N., Yoshitomi, H., Tanida, S., Ishikawa, M., Kasahara, T., Shibuya, H., Fujii, N., Nagasawa, T., Nakamura, T. and Ito, H. Stromal cell-derived factor 1 regulates the actin organization of chondrocytes and chondrocyte hypertrophy. PLoS ONE 7: e37163 (2012).
Ryan, G. L., Petroccia, H. M., Watanabe, N. and Vavylonis, D. Excitable actin dynamics in lamellipodial protrusion and retraction. Biophys. J. 102: 1493-1502 (2012)
Ryan, G. L., Watanabe, N. and Vavylonis, D. A review of models of fluctuating protrusion and retraction patterns at the leading edge of motile cells. (Review) Cytoskeleton (Hoboken) 69: 195-206 (2012).
Millius, A., Watanabe, N. and Weiner, O.D. Diffusion, capture, and recycling of SCAR/WAVE and Arp2/3 complexes observed in cells with single-molecule imaging. J. Cell Sci. 125: 1165-1176 (2012).
Sakamoto, S., Ishizaki, T., Okawa, K., Watanabe, S., Arakawa, A., Watanabe, N. and Narumiya, S. Liprin-α controls stress fiber formation by binding to mDia and regulating its membrane localization. J. Cell Sci. 125: 108-120 (2012).
Maruoka, M., Sato, M., Yuan, Y., Ichiba, M., Fujii, R., Ogawa, T., Ishida-Kitagawa, N., Takeya, T. and Watanabe, N. Abi-1-bridged tyrosine phosphorylation of VASP by Abelson kinase impairs association of VASP to focal adhesions and regulates leukemic cell adhesion. Biochem. J. 441: 889-899 (2012).
2011
Sato, Y., Watanabe, N., Fukushima, N., Mita, S. and Hirata, T. Actin-independent behavior and membrane deformation exhibited by the four-transmembrane protein M6a. PLoS ONE 6: e26702 (2011).
Kiuchi, T., Nagai, T., Ohashi, K., Watanabe, N. and Mizuno, K. Live-cell imaging of G-actin dynamics using sequential FDAP. (review) Bioarchitecture 1, 1-5 (2011)
Smith, M.B., Karatekin, E., Gohlke, A., Mizuno, H., Watanabe, N. and Vavylonis, D. Interactive, computer-assisted tracking of speckle trajectories in fluorescence microscopy: application to actin polymerization and membrane fusion. Biophys. J. 101: 1794-1804 (2011).
Kiuchi, T., Nagai, T., Ohashi, K. and Mizuno, K. Measurements of spatiotemporal changes in G-actin concentration reveal its effect on stimulus-induced actin assembly and lamellipodium extension. J. Cell Biol., 193, 365-380 (2011).
Mizuno, H., Higashida, C., Yuan, Y., Ishizaki, T., Narumiya, S. and Watanabe, N. Rotational movement of the formin mDia1 along the double helical strand of an actin filament. Science, 331: 80-83 (2011).
2010 and before (selected)
Watanabe N. Inside view of cell locomotion through single-molecule: fast F-/G-actin cycle and G-actin regulation of polymer restoration. (review) Proc. Jpn. Acad. B. Phys. Biol. Sci. 86: 62-83. (2010)
Monypenny, J., Zicha, D., Higashida, C., Oceguera-Yanez, F., Narumiya, S. and Watanabe, N. Cdc42 and Rac family GTPases regulate mode and speed but not direction of primary fibroblast migration during PDGF-dependent chemotaxis. Mol. Cell. Biol. 29: 2730-2747 (2009).
Tsuji, T., Miyoshi, T., Higashida, C., Narumiya, S. and Watanabe, N. An order of magnitude faster AIP1-associated actin disruption than nucleation by the Arp2/3 complex in lamellipodia. PLoS ONE 4: e4921 (2009).
Fujita, A., Shishido, T., Yuan, Y., Inamoto, E., Narumiya, S. and Watanabe, N. Imatinib mesylate (STI571)-induced cell edge translocation of kinase-active and kinase-defective Abelson kinase: requirements of myristoylation and src homology 3 domain. Mol. Pharmacol. 75: 75-84 (2009).
Higashida, C., Suetsugu, S., Tsuji, T., Monypenny, J., Narumiya, S. and Watanabe, N. G-actin regulates rapid induction of actin nucleation by mDia1 to restore cellular actin polymers. J. Cell Sci. 121: 3403-3412 (2008).
Shimada, T., Toriyama, M., Uemura, K., Kamiguchi, H., Sugiura, T., Watanabe, N., and Inagaki, N. Shootin1 interacts with actin retrograde flow and L1-CAM to promote axon outgrowth. J. Cell Biol. 181: 817-829 (2008).
Watanabe, S., Ando, Y., Yasuda, S., Hosoya, H., Watanabe, N., Ishizaki, T., and Narumiya, S. mDia2 induces the actin scaffold for the contractile ring and stabilizes its position during cytokinesis in NIH 3T3 cells. Mol. Biol. Cell 19: 2328-2338 (2008).
Miyoshi T, Tsuji T, Higashida C, Hertzog M, Fujita A, Narumiya S, Scita G, Watanabe N. Actin turnover-dependent fast dissociation of capping protein in the dendritic nucleation actin network: evidence of frequent filament severing. J. Cell Biol. 175: 947-955 (2006).
Watanabe, N. and Higashida, C. Formins: Processive cappers of growing actin filaments. (Review) Exp. Cell Res. 301: 16-22, (2004)
Higashida C. Miyoshi T., Fujita A., Oceguera-Yanez F., Monypenny J., Andou Y., Narumiya S. and Watanabe N. Actin polymerization-driven molecular movement of mDia1 in living cells. Science 303: 2007-2010 (2004).
Watanabe N. and Mitchison T.J. Single-molecule speckle analysis of actin filament turnover in lamellipodia. Science 295: 1083-1086 (2002)
Kato, T., Watanabe, N., Morishima, Y., Fujita, A., Ishizaki, T. and Narumiya, S. Localization of a mammalian homolog of diaphanous, mDia1, to the mitotic spindle in HeLa cells. J. Cell Sci. 114: 775-784 (2001).
Watanabe N, Kato T, Fujita A, Ishizaki T, Narumiya S. Cooperation between mDia1 and ROCK in Rho-induced actin reorganization. Nat. Cell Biol. 1: 136-143 (1999)
Watanabe, N., Madaule, P., Reid, T., Ishizaki, T., Watanabe, G., Kakizuka, A., Saito, Y., Nakao, K., Jockusch, B.M. and Narumiya, S. p140mDia, a mammalian homolog of Drosophila diaphanous, is a target protein for Rho small GTPase and is a ligand for profilin. EMBO J. 16: 3044-3056 (1997).
Narumiya, S. and Watanabe, N. Migration without a clutch. (News and Views) Nat. Cell Biol. 11: 1394-1396 (2009)
Watanabe, N. and Higashida, C. A possible role of homeostasis between monomeric and filamentous actin in filament nucleation revealed by pharmacokinetic modeling. Systems Biology The Challenge of Complexity. Springer 143-149 (2009)